Hepa 1-6 (ATCC® CRL-1830)

Organism: Mus musculus, mouse  /  Tissue: liver  /  Disease: hepatoma

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Organism Mus musculus, mouse
Tissue liver
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease hepatoma
Strain C57L
Applications
This line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Derivation
This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.
Genes Expressed
albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Growth Conditions: The cells can also be propagated in a serum free medium consisting of 3 parts Dulbecco's modified Eagle's medium, 1 part Waymouth's MAB 87/3 medium and 30 nM selenium.
Name of Depositor GJ Darlington
References

Darlington GJ. Liver cell lines. Methods Enzymol. 151: 19-38, 1987. PubMed: 3431441

Darlington GJ, et al. Expression of liver phenotypes in cultured mouse hepatoma cells. J. Natl. Cancer Inst. 64: 809-819, 1980. PubMed: 6102619

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Darlington GJ. Liver cell lines. Methods Enzymol. 151: 19-38, 1987. PubMed: 3431441

Darlington GJ, et al. Expression of liver phenotypes in cultured mouse hepatoma cells. J. Natl. Cancer Inst. 64: 809-819, 1980. PubMed: 6102619