LMH/2A (ATCC® CRL-2118)

Organism: Gallus gallus, chicken  /  Tissue: hepatocellular carcinoma; liver; chemically induced; transfected with chicken estrogen receptor gene  /  Disease: hepatocellular carcinoma

Permits and Restrictions

View Permits

Organism Gallus gallus, chicken
Tissue hepatocellular carcinoma; liver; chemically induced; transfected with chicken estrogen receptor gene
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease hepatocellular carcinoma
Strain Leghorn
Karyotype Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines: triploid; modal number = 116; six marker chromosomes
Derivation

LMH/2A is a stably transfected cell line constructed in 1989 by R. Binder from the LMH hepatocellular carcinoma cell line (see ATCC CRL-2117) by cotransfection with the plasmid pRSVCEO (containing the chicken estrogen receptor gene driven by the Rous sarcoma promoter) and the pRSVNEO plasmid (which confers G418 resistance).


Receptor Expression
estrogen receptor, positive
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Comments
LMH/2A cells express high levels of chicken estrogen receptor.

Apolipoprotein (apoII) gene expression is increased by estrogen 300-fold compared with levels in the low receptor expression parent LMH line.

This cell line is estrogen responsive for the expression of endogenous yolk protein genes, and permits the analysis of estrogen responsive genes involved in the transport of yolk proteins and other nutrients to the oocyte.

These genes include apolipoprotein B and apoII as well as vitellogenin.

NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.
To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes, and remove the liquid.
Vessels prepared in this fashion may be used immediately.
Complete Growth Medium Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution. Allow the flasks to sit at room temperature (or incubate at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Flasks must be coated with 0.1% gelatin.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days

Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor R Binder, DL Williams
References

Binder R, et al. Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990. PubMed: 2330000

Kawaguchi T, et al. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463, 1987. PubMed: 3607775

Sensel MG, et al. Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. Mol. Cell. Biol. 14: 1733-1742, 1994. PubMed: 8114707

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Binder R, et al. Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990. PubMed: 2330000

Kawaguchi T, et al. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463, 1987. PubMed: 3607775

Sensel MG, et al. Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. Mol. Cell. Biol. 14: 1733-1742, 1994. PubMed: 8114707