FL83B (ATCC® CRL-2390)

Organism: Mus musculus, mouse  /  Cell Type: hepatocyte  /  Tissue: liver  / 

Organism Mus musculus, mouse
Tissue liver
Cell Type hepatocyte
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 15 to 17 days gestation
Storage Conditions liquid nitrogen vapor phase
Karyotype The cells are aneuploid. Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Derivation
FL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal mouse.
Genes Expressed
cholesterol; glycogen
Comments
These cells are reported to store glycogen and actively synthesize cholesterol. They have also been reported to be able to produce serum proteins.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C

Subculture Ratio: 1:4 to 1:6
Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor EH Leiter
Year of Origin 1969
References

Breslow JL, et al. Characterization of the mouse liver cell line FL83B. Exp. Cell Res. 78: 441-453, 1973. PubMed: 4572697

FL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal mouse. A culture submitted to the ATCC in May 1988was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM -Cycline. The cells were assayed for mycoplasma by the Hoechst stain, PCR and the standard culture test after a six-week period following treatment. All tests were negative.

Basic Documentation
References

Breslow JL, et al. Characterization of the mouse liver cell line FL83B. Exp. Cell Res. 78: 441-453, 1973. PubMed: 4572697

FL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal mouse. A culture submitted to the ATCC in May 1988was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM -Cycline. The cells were assayed for mycoplasma by the Hoechst stain, PCR and the standard culture test after a six-week period following treatment. All tests were negative.