PLC/PRF/5 (ATCC® CRL-8024)

Organism: Homo sapiens, human  /  Cell Type: Alexander cells  /  Tissue: liver  /  Disease: hepatoma

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Organism Homo sapiens, human
Tissue liver
Cell Type Alexander cells
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain hepatitis B]

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease hepatoma
Genes Expressed
hepatitis virus B surface antigen (HBsAg)
Cellular Products
hepatitis virus B surface antigen (HBsAg)
Comments
The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline.
The cells secrete HBsAg.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 8
TPOX: 8
vWA: 15,16
Name of Depositor WJ McAleer
U.S. Patent Number
References

Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed: 223321

MacNab GM, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer 34: 509-515, 1976. PubMed: 187208

Skelly J, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138

Knowles B. et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209(4455): 497-499, 1980. PubMed: 6248960

Alexander J, et al. Adaptation of cells derived from human malignant tumours to growth in vitro. S. Afr. J. Med. Sci. 41(2):89-98, 1976. PubMed:184551

Gilligan A, et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed: 1315281

Alexander JJ, et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J. 50: 2124-2128, 1976. PubMed: 63998

Cross References

Nucleotide (GenBank) : X75311 H.sapiens /Hepatitis B virus fusion mRNA for mevalonate kinase.

Nucleotide (GenBank) : X77700 H.sapiens / Hepatitis B virus (A4.0') DNA for mevalonate kinase (partial).

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed: 223321

MacNab GM, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer 34: 509-515, 1976. PubMed: 187208

Skelly J, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138

Knowles B. et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209(4455): 497-499, 1980. PubMed: 6248960

Alexander J, et al. Adaptation of cells derived from human malignant tumours to growth in vitro. S. Afr. J. Med. Sci. 41(2):89-98, 1976. PubMed:184551

Gilligan A, et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed: 1315281

Alexander JJ, et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J. 50: 2124-2128, 1976. PubMed: 63998