DMS 153 (ATCC® CRL-2064)

Organism: Homo sapiens, human  /  Tissue: lung  /  Disease: carcinoma; small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Tissue lung
Product Format frozen
Culture Properties monolayer of adherent clusters
Biosafety Level 1
Disease carcinoma; small cell lung cancer
Age 44 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was established from metastatic cells from liver tissue taken at autopsy of a patient with small cell carcinoma of the lung.
Clinical Data The patient had received prior therapy with cytoxan and methotrexate.
Antigen Expression
Leu 7 +; My23 +; CD11b +
Receptor Expression
Bombesin; epidermal growth factor (EGF); complement (CR3)
Genes Expressed
Adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; calcitonin gene related peptide (CGRP); oxytocin - neurophysin (OT-NP); 17 beta estradiol,Leu 7 +; My23 +; CD11b +
Tumorigenic Yes
Effects
Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments
The cells express HLA class I and class II antigens.
Complete Growth Medium Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:2 to 1:3
Medium Renewal: Twice per week

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions Keep cells heavy and subculture often.
Name of Depositor OS Pettengill, G Sorenson
References

Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Note: These cells are distributed subject to the following:

  1. This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College.
  2. Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675.
  3. In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.

References

Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.