NCI-H378 [H378] (ATCC® CRL-5808)

Organism: Homo sapiens, human  /  Disease: stage E,carcinoma; classic small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Disease stage E,carcinoma; classic small cell lung cancer
Age 66 years
Gender female
Ethnicity Caucasian
Applications
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from cells recovered from pleural effusion taken from a patient after chemotherapy.
NCI-H378 expresses elevated levels of the 4 biochemical markers of SCLC: neuron specific enolase, the brain isoenzyme of creatine kinase, L-dopa carboxylase and bombesin-like immunoreactivity.
The cells express the c-kit gene as well as the L-myc gene, and L-myc is amplified.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from cells recovered from pleural effusion taken from a patient after chemotherapy.
Clinical Data
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from cells recovered from pleural effusion taken from a patient after chemotherapy.
female
Caucasian
66 years
Oncogene c-kit +; L-myc +
Genes Expressed
c-kit +; L-myc +
Tumorigenic Yes
Effects
Yes, the cells produce tumors in athymic nude mice
Comments
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from cells recovered from pleural effusion taken from a patient after chemotherapy.
NCI-H378 expresses elevated levels of the 4 biochemical markers of SCLC: neuron specific enolase, the brain isoenzyme of creatine kinase, L-dopa carboxylase and bombesin-like immunoreactivity.
The cells express the c-kit gene as well as the L-myc gene, and L-myc is amplified.
The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 12
D5S818: 11,12
D7S820: 12
THO1: 6,9.3
TPOX: 8,9
vWA: 16,19
Name of Depositor AF Gazdar, JD Minna
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Plummer H, et al. c-myc expression correlates with suppression of c-kit protooncogene expression in small cell lung cancer cell lines. Cancer Res. 53: 4337-4342, 1993. PubMed: 7689933

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Plummer H, et al. c-myc expression correlates with suppression of c-kit protooncogene expression in small cell lung cancer cell lines. Cancer Res. 53: 4337-4342, 1993. PubMed: 7689933

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.