NCI-H220 [H220] (ATCC® CRL-5825)

Organism: Homo sapiens, human  /  Disease: stage E,carcinoma; classic small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Product Format frozen
Culture Properties suspension, floating aggregates of round cells with some attached cells
Biosafety Level 1
Disease stage E,carcinoma; classic small cell lung cancer
Age 51 years
Gender male
Ethnicity Black
Applications
The line was established in February 1980.
Derivation
The line was established in February 1980.
Clinical Data
The tissue donor was a non-smoker.
male
Black
51 years
Comments
The line was established in February 1980.
The tissue donor was a non-smoker.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 3 to 5 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1980
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.