SVEC4-10EE2 (ATCC® CRL-2167)

Organism: Mus musculus, mouse  /  Cell Type: endothelial, SV40 transformed  / 

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Organism Mus musculus, mouse
Cell Type endothelial, SV40 transformed
Product Format frozen
Morphology cobblestone
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult
Gender male
Strain C3H/HeJ
Applications
SVEC4-10EE2 is an endothelial cell line derived from the same solid tumor as SVEC4-10EHR1 (see CRL-2161) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181).
The cells were cloned in 1989 by limiting dilution, and are distinguished by their inability to differentiate on a synthetic basement-like membrane.
The cells exhibit a stable cobblestone morphology in contrast to its sibling from the same tumor (SVEC4-10EHR1).
Storage Conditions liquid nitrogen vapor phase
Derivation
SVEC4-10EE2 is an endothelial cell line derived from the same solid tumor as SVEC4-10EHR1 (see CRL-2161) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181).
Clinical Data
male
Antigen Expression
H-2 K; Factor VIII related antigen; VCAM
Receptor Expression
high affinity receptors for low density lipoprotein (LDL)
Genes Expressed
H-2 K; Factor VIII related antigen; VCAM
Tumorigenic Yes
Effects
Yes, the cells induce spindle tumors with some of the histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks
Comments
SVEC4-10EE2 is an endothelial cell line derived from the same solid tumor as SVEC4-10EHR1 (see CRL-2161) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181).
The cells were cloned in 1989 by limiting dilution, and are distinguished by their inability to differentiate on a synthetic basement-like membrane.
They form tube-like structures in the presence of suramin, a drug which blocks growth factor receptors.
The cells exhibit a stable cobblestone morphology in contrast to its sibling from the same tumor (SVEC4-10EHR1).
The cells express the cell surface major histocompatibility complex class I antigen, H-2 k of the parental cell line, and express Factor VIII related antigen, high affinity receptors for low density lipoprotein and vascular cell adhesion molecule (VCAM).
The cells stain positively for SV40 T antigen.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Subculturing
Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor KA O'Connell
Year of Origin 1989
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299