IP2-E4 (ATCC® CRL-2171)

Organism: Mus musculus, mouse  /  Cell Type: endothelial, SV40 transformed  / 

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Organism Mus musculus, mouse
Cell Type endothelial, SV40 transformed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2
Age adult
Gender male
Strain C3H/HeJ
Applications
The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
IP2-E4 cells are sensitive to lysis by activated macrophage as measured in the chromium release assay.
They do not express high levels of vascular cell adhesion molecule (VCAM) constitutively however, stimulation with TNF alpha caused an up regulation of VCAM expression.
This clone retains the ability to differentiate on a synthetic basement-like membrane.
Derivation
The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
Clinical Data
male
Antigen Expression
H-2 K; VCAM
Genes Expressed
H-2 K; VCAM
Tumorigenic Yes
Effects
Yes, the cells induced spindle tumors in nude mice with some of the histopathologic characteristics of human Kaposi Sarcoma after a shortened latency period of approximately 2 weeks
Comments
The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
The line was cloned in 1992 by limiting dilution.
IP2-E4 cells are sensitive to lysis by activated macrophage as measured in the chromium release assay.
The cells express the cell surface major histocompatibility complex class I antigen, H-2 k of the parental cell line.
They do not express high levels of vascular cell adhesion molecule (VCAM) constitutively however, stimulation with TNF alpha caused an up regulation of VCAM expression.
This clone retains the ability to differentiate on a synthetic basement-like membrane.
The cells stain positively for SV40 T antigen.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor KA O'Connell
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299