SU-DHL-1 (ATCC® CRL-2955)

Organism: Homo sapiens, human  /  Cell Type: histiocytic  /  Tissue: lymph node
Derived from Metastatic Site: peritoneal effusion

 /  Disease: large cell lymphoma; diffuse histiocytic lymphoma

Organism Homo sapiens, human
Tissue lymph node
Derived from Metastatic Site: peritoneal effusion

Cell Type histiocytic
Product Format frozen
Morphology lymphoblast-like
Culture Properties suspension
Biosafety Level 1
Disease large cell lymphoma; diffuse histiocytic lymphoma
Age 10 years
Gender Male
Ethnicity Caucasian
Applications The availability of a permanently established human neoplastic cell line as the source of this type C RNA virus should greatly facilitate studies of its biological significance. Evidence has recently been obtained that the virus can induce proliferation of normal human monocytes and histiocytes in vitro.
Storage Conditions liquid nitrogen vapor phase
Karyotype 88 chromosomes; t(2;5)(p23;q35) translocation
Images CRL-2955 Micrograph
Clinical Data 10 years
Caucasian
Male
Antigen Expression Monocyte Marker: CD163+
Lymphoid Marker: CD45-
Progenitor Markers: CD10-, CD34-
Activation Markers: CD30+, CD25+, CD70+, CD71+, CD80-, HLA-DR+, CD45-
T-Cell Markers: CD2-, CD3-, CD4-, CD5+, CD7-, CD8-
B-Cell Markers: CD19-, CD20-, CD21-, CD22-
Myelomonocytic Markers: CD11b-, CD11c-, CD13-, CD14-, CD15-, CD33-
Receptor Expression CFS-1 expressed
IL-1-R, IL-2-Rα, IL-6-R, TNFα-R, & c-fms expressed
Surface Receptors: Fc, IgMEAC, IgGEA, & E not expressed
Oncogene c-fms (proto-oncogene), bcl-6+(c-onc)
Genes Expressed Fusion gene NPM-ALK (p80)+
anaplastic lymphoma kinase (ALK)+

Ig, not expressed


Comments The cells are surface Ig negative (sIG-).
The cells are non-specific esterase, acid phosphatase and oil red O positive.
The cells are periodic acid Schiff negative.
These cells phagocytose Candida albicans and latex particles.
The cells are reported to be very weakly E - rosette positive.
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 104 to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 106 and 1.5 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:12 is recommended.
Interval: As needed.
Medium renewal: every 2 to 4 days
Cryopreservation Storage temperature: liquid nitrogen vapor phase
Freeze medium: Complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 11, 12
D13S317: 9, 13
D7S820: 10, 13
D16S539: 11, 12
vWA: 15, 17
THO1: 6, 7
Amelogenin: X Y
TPOX: 8
CSF1PO: 12
Population Doubling Capacity infinite
Name of Depositor A Epstein
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Basic Documentation
Other Documentation
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794