SU-DHL-2 (ATCC® CRL-2956)

Organism: Homo sapiens, human  /  Cell Type: histiocytic  /  Tissue: lymph node
Derived from Metastatic Site: peritoneal effusion
 /  Disease: large cell lymphoma; diffuse histiocytic lymphoma

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Organism Homo sapiens, human
Tissue lymph node
Derived from Metastatic Site: peritoneal effusion
Cell Type histiocytic
Product Format frozen
Morphology Lymphoblast-like
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Disease large cell lymphoma; diffuse histiocytic lymphoma
Age 73 years
Gender Female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Most of these cells were hyperdiploid, with a sharp modal number of 51 chromosomes; occasional cells in the hypertetraploid range were also seen. A minute marker chromosome was consistently observed, and increased numbers of chromosomes were seen in the A, B, C, and F groups.
Images CRL-2956 Cell Micrograph
Clinical Data 73 years
Caucasian
Female
Comments The cells are non-specific esterase and acid phosphatase positive.
The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-).
The cells are surface Ig negative (sIG-).
The cells are reported to be E - rosette negative.
ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. An inoculum of 1 X 105 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Storage temperature: liquid nitrogen vapor phase

Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Culture Conditions Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 13
D13S317: 11, 12
D7S820: 11, 13
D16S539: 14
vWA: 17
THO1: 6, 9
TPOX: 8
CSF1PO: 12
Amelogenin: X
Name of Depositor A Epstein
Year of Origin 1974
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794