SU-DHL-8 (ATCC® CRL-2961)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: lymph node; derived from metastatic site: peritoneal effusion  /  Disease: Large cell lymphoma

Organism Homo sapiens, human
Tissue lymph node; derived from metastatic site: peritoneal effusion
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast-like
Culture Properties suspension, grow as single cells and small clusters
Biosafety Level 1
Disease Large cell lymphoma
Age 59 years
Gender Male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images CRL-2961 Micrograph
Clinical Data 59 years
Caucasian
Male
Receptor Expression Fc (not expressed), IgMEAC (not expressed), IgGEA (not expressed), E (not expressed)
Genes Expressed Ig +; IgM-, IgG-, IgA-, IgD-, Lambda-, Kappa-
Comments

The depositor states that this cell line is negative for Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
This cell line is negative for Canadida ingestion.
This is a null cell line (absence of all known markers) based on the classification of diffuse histiocytic lymphomas.
These cells exhibit no response to LY294002-induced apoptosis.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. Subculture the cells when cell density is ~ 1.0 x 106 to 2.5 x 106 cells/mL for higher viability. An inoculum of 1.0 x 105 to 4.0 x 105 cells/mL is recommended.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended.
Medium renewal: Every 2 to 4 days (depending on cell density)
Cryopreservation Storage temperature: liquid nitrogen vapor phase
Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 11, 13
D13S317: 11, 13
D7S820: 8
D16S539: 12
CSF1PO: 11, 12
vWA: 15, 19
THO1: 6, 9
TPOX: 8
Amelogenin: X
Year of Origin 1978
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Basic Documentation
Other Documentation
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220