Hs 505.T (ATCC® CRL-7306)

Organism: Homo sapiens, human  /  Disease: chronic lymphocytic leukemia (CLL)

Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease chronic lymphocytic leukemia (CLL)
Gender female
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
Images
Clinical Data
Patient had Kaposi's sarcoma.
female
Black
Comments
Patient had Kaposi's sarcoma.
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:Volumes used in this protocol are for 75 sq. cm. flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37.0°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and centrifuge at approximately 125 xg for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  7. Incubate cultures at 37.0°C.
Subcultivation ratio: 1:2 to 1:3 every 7 to 10 days
Medium renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 10,13
D5S818: 12
D7S820: 8,10
THO1: 8,9
TPOX: 6,8
vWA: 15
Name of Depositor Naval Biosciences Laboratory