G-8 (ATCC® CRL-1456)

Organism: Mus musculus, mouse  /  Cell Type: myoblast myoblast  / 

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Organism Mus musculus, mouse
Cell Type myoblast myoblast
Product Format frozen
Morphology myoblast
Culture Properties adherent
Biosafety Level 1
Age fetus fetus
Karyotype modal number = 66
Receptor Expression
acetylcholine
Genes Expressed
myosin
Cellular Products
myosin
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, semi-solid media
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Subculturing
Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks. Differentiation is enhanced by reducing the sera to 2% each.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Culture Conditions
Temperature: 37.0°C
Population Doubling Time 25 to 27 hrs
Name of Depositor J Peacock
References

Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191

The cells are highly responsive to acetylcholine.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191

The cells are highly responsive to acetylcholine.