L8 (ATCC® CRL-1769)

Organism: Rattus norvegicus, rat  /  Cell Type: myoblast myoblast  /  Disease: Carcinogen

Organism Rattus norvegicus, rat
Cell Type myoblast myoblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Carcinogen
Age newborn
Applications
transfection host
Derivation
This line was originally isolated by D. Yaffe in 1969 from primary rat skeletal muscle cultures;
Genes Expressed
creatine phosphokinase (CPK); myosin
Cellular Products
creatine phosphokinase (CPK); myosin
Comments
This line was originally isolated by D. Yaffe in 1969 from primary rat skeletal muscle cultures;
unlike the L6 cell line (ATCC CRL-1458) no carcinogen was used to establish the L8 line;
upon becoming confluent, L8 will fuse to form cross striated multinucleated muscle fibers;
it is recommended that early passages be preserved in liquid nitrogen and that the line be recloned periodically and reselected for progeny that have the ability to fuse;
it is important that the cells be subcultured when the flask is about 60% confluent.
The myoblastic population will be depleted if the cultures are allowed to become confluent since most of the cells will fuse into nondividing syncytia.
To avoid this, one must subculture before before the cultures become confluent and should reclone periodically and select myoblastic clones.
Complete Growth Medium A 4:1 mixture of Dulbecco's modified Eagle's medium and Medium 199, 89%; chicken embryo extract, 1%; horse serum, 10%
Subculturing
Subcultivation Ratio: An inoculation density of 4000 viable cells per sq. cm. of flask or dish surface area is recommended
Medium Renewal: Twice per week
Remove medium, rinse the cell sheet once with fresh trypsin (0.25%), EDTA (0.03%) solution, remove trypsin and allow the flask to sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks at a density of 2000 cells/sq. cm.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor B Paterson
Passage History
it is recommended that early passages be preserved in liquid nitrogen and that the line be recloned periodically and reselected for progeny that have the ability to fuse;
References

Richler C, Yaffe D. The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev. Biol. 23: 1-22, 1970. PubMed: 5481965

Yaffe D, Saxel O. A myogenic cell line with altered serum requirements for differentiation. Differentiation 7: 159-166, 1977. PubMed: 558123

Basic Documentation
References

Richler C, Yaffe D. The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev. Biol. 23: 1-22, 1970. PubMed: 5481965

Yaffe D, Saxel O. A myogenic cell line with altered serum requirements for differentiation. Differentiation 7: 159-166, 1977. PubMed: 558123