mh [mocha] (ATCC® CRL-2709)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  / 

Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 1 to 3 days newborn
Storage Conditions lliquid nitrogen vapor phase
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: lliquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor AA Peden
References

Peden AA, et al. Assembly and function of AP-3 complexes in cells expressing mutant subunits. J. Cell Biol. 156: 327-336, 2002. PubMed: 11807095

. Mutation in AP-3 delta in the mocha mouse links endosomal transport to storage deficiency in platelets, melanosomes, and synaptic vesicles.. Neuron 21: 111-122, 1998. PubMed: 9697856

The mh cell line was derived from primary fibroblasts taken from muscle of homozygous mocha (mh/mh) mice. As a result of the mocha mutation, the fibroblasts are deficient in the delta subunit of the adaptor-like protein complex, AP-3. The cell line can be used to investigate both the assembly of AP-3 complexes and AP-3 function, and may serve as a model for Hermansky-Pudlak syndrome (HPS), a platelet storage pool deficiency syndrome.

Basic Documentation
References

Peden AA, et al. Assembly and function of AP-3 complexes in cells expressing mutant subunits. J. Cell Biol. 156: 327-336, 2002. PubMed: 11807095

. Mutation in AP-3 delta in the mocha mouse links endosomal transport to storage deficiency in platelets, melanosomes, and synaptic vesicles.. Neuron 21: 111-122, 1998. PubMed: 9697856

The mh cell line was derived from primary fibroblasts taken from muscle of homozygous mocha (mh/mh) mice. As a result of the mocha mutation, the fibroblasts are deficient in the delta subunit of the adaptor-like protein complex, AP-3. The cell line can be used to investigate both the assembly of AP-3 complexes and AP-3 function, and may serve as a model for Hermansky-Pudlak syndrome (HPS), a platelet storage pool deficiency syndrome.