RTG-2 (ATCC® CCL-55)

Organism: Oncorhynchus mykiss, trout, rainbow  / 

Permits and Restrictions

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Organism Oncorhynchus mykiss, trout, rainbow
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Gender male and female mixed
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male and female mixed
Virus Resistance
poliovirus 1
Comments
Maximum growth rate occurs at 22°C.
Above 26°C the cells do not survive.
Cells will grow at temperatures as low as 4°C.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Once per week
Harvest cells with cold (less than 24C) 0.25% trypsin or 0.02% versene (or a mixture of the two). Cultures may be established with as few as 3 X 10 exp3 cells/ml.
Name of Depositor K Wolf
References

. . Science 135: 1065, 1962.

Officer JE. Ability of a fish cell line to support the growth of mammalian viruses. Proc. Soc. Exp. Biol. Med. 116: 190-194, 1964. PubMed: 14200103

Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741

Cross References

Nucleotide (GenBank) : U82129 Oncorhynchus mykiss Y1 RNA, partial sequence.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Science 135: 1065, 1962.

Officer JE. Ability of a fish cell line to support the growth of mammalian viruses. Proc. Soc. Exp. Biol. Med. 116: 190-194, 1964. PubMed: 14200103

Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741