Antheraea cells (ATCC® CCL-80)

Organism: Antheraea eucalypti, moth  / 

Organism Antheraea eucalypti, moth
Product Format frozen
Morphology spindle, round and crescent shaped cells
Culture Properties suspension
Biosafety Level 1
Gender female
Applications
This cell line derived from ovarian tissues of the moth, Antheraea eucalypti, by Grace in 1962, constituted the first true line of arthropod cells established in cell culture.
Derivation
This cell line derived from ovarian tissues of the moth, Antheraea eucalypti, by Grace in 1962, constituted the first true line of arthropod cells established in cell culture. Because of the difficulty and expense in obtaining significant volumes of lepidopteran hemolymph, the Antheraea cells were adapted to hemolymph-free culture medium by Yunker, Vaughn and Cory . The cell line was grown in Grace's insect tissue culture medium supplemented with 10% FBS (heat-inactivated), 10% whole chicken egg ultrafiltrate and 1% bovine plasma albumin (fraction V). The cells grow predominantly in suspension although some cells adhere to the vessel walls. Yunker and Cory found the adapted Antheraea cells were able to support the growth of a number of arboviruses.
Clinical Data
female
Virus Resistance
poliovirus 1; herpes simplex; vaccinia
Comments
This cell line derived from ovarian tissues of the moth, Antheraea eucalypti, by Grace in 1962, constituted the first true line of arthropod cells established in cell culture. Because of the difficulty and expense in obtaining significant volumes of lepidopteran hemolymph, the Antheraea cells were adapted to hemolymph-free culture medium by Yunker, Vaughn and Cory . The cell line was grown in Grace's insect tissue culture medium supplemented with 10% FBS (heat-inactivated), 10% whole chicken egg ultrafiltrate and 1% bovine plasma albumin (fraction V). The cells grow predominantly in suspension although some cells adhere to the vessel walls. Yunker and Cory found the adapted Antheraea cells were able to support the growth of a number of arboviruses.
Complete Growth Medium Grace's insect medium, 79%; heat-inactivated fetal bovine serum, 10%; whole egg ultrafiltrate, 10%; bovine plasma albumin (fraction V), 1%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:12 is recommended
Medium Renewal: At the time of subcultivation (approximately every 6 days)
The cells grow in suspension and subcultures are prepared by diluting 0.5 ml of cell suspension with 4.5 ml of fresh culture medium and dispensing into a new flask. Culture at 23C to 25C.
Name of Depositor JL Vaughn
Year of Origin 1962
References

. . Nature 195: 1, 1962.

Yunker CE, et al. Adaptation of an insect cell line (Grace's Antheraea cells) to medium free of insect hemolymph. Science 155: 1565-1566, 1967. PubMed: 6020481

Yunker CE, Cory J. Infection of Grace's Antheraea cells with Arboviruses. Am. J. Trop. Med. Hyg. 17: 889-893, 1968. PubMed: 5726135

Basic Documentation
References

. . Nature 195: 1, 1962.

Yunker CE, et al. Adaptation of an insect cell line (Grace's Antheraea cells) to medium free of insect hemolymph. Science 155: 1565-1566, 1967. PubMed: 6020481

Yunker CE, Cory J. Infection of Grace's Antheraea cells with Arboviruses. Am. J. Trop. Med. Hyg. 17: 889-893, 1968. PubMed: 5726135