TOV-21G (ATCC® CRL-11730)

Organism: Homo sapiens, human  /  Tissue: ovary  /  Disease: grade 3, stage III,primary malignant adenocarcinoma; clear cell carcinoma

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Organism Homo sapiens, human
Tissue
ovary
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease grade 3, stage III,primary malignant adenocarcinoma; clear cell carcinoma
Age 62 years
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype 47, XX, +10 RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
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Derivation
This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer.
Clinical Data
female
Oncogene p53 + (wild type)
Genes Expressed
keratin
Cellular Products
keratin
Tumorigenic Yes
Effects
Yes, form colonies in soft agar
Yes, the cells are tumorigenic in nude mice
Comments
Like OV-90 (ATCC CRL-11732), the OV-21G (ATCC CRL-11730) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731) cell line does not exhibit a deletion at chromosome 3p24.
Complete Growth Medium The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • Subculturing
    Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
    Medium Renewal: Every 3 to 4 days
    Cryopreservation
    Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage Temperature: Liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    STR Profile
    Amelogenin: X
    CSF1PO: 13,15
    D13S317: 11,12
    D16S539: 10,12
    D5S818: 12,13
    D7S820: 12
    THO1: 7,9.3
    TPOX: 8,11
    vWA: 17
    Name of Depositor University of Montreal
    Year of Origin 1991
    References

    Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998

    Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

    Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    Other Documentation
    References

    Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998

    Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

    Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993