Sf9 (ATCC® CRL-1711)

Organism: Spodoptera frugiperda, fall armyworm  /  Tissue: ovary  / 

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Organism Spodoptera frugiperda, fall armyworm
Tissue
ovary
Product Format frozen
Morphology epithelial
Culture Properties mixed: adherent/suspension
Biosafety Level 1
Age pupa
Gender female
Applications
This line can be used to replicate baculovirus expression vectors. This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
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Clinical Data
female
Virus Susceptibility Nuclear polyhedrosis virus, Autographa californica
St. Louis encephalitis virus , St. Louis encephalitis virus
Comments
It is important to use the medium described below.
Complete Growth Medium The base medium for this cell line is Grace's Insect Medium Supplemented (GIBCO/Invitrogen Cat. No. 11605-094 or equivalent). To make the complete growth medium, add the following components to the base medium: heat-inactivated insect cell culture tested fetal bovine serum to a final concentration of 10%.
Subculturing
Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand (the latter is only preferable when working with larger flasks).
Subcultivation Ratio: A subcultivation ratio of 1:5 or greater is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 28°C
Growth Conditions: The recommended media are formulated for use without CO2. Omission of the yeastolate or lactalbumin hydrolysate will lead to poor performance by this line.
Name of Depositor G Smith, C Cherry, MD Summers
Year of Origin 1983
References

Vaughn JL, et al. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13: 213-217, 1977. PubMed: 68913

Zhang PF, et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-213, 1994. PubMed: 7811453

Smith GE, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985. PubMed: 3878519

Hall MR, Gibson W. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separately cloned and coexpressed in eukaryotic cells. J. Virol. 70: 5395-5404, 1996. PubMed: 8764050

Bakker A, et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J. Virol. 70: 5511-5518, 1996. PubMed: 8764063

Gibson W, et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J. Virol. 70: 7454-7461, 1996. PubMed: 8892863

Filtz TM, et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J. Biol. Chem. 49: 31121-31126, 1996. PubMed: 8940109

Lindorfer MA, et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J. Biol. Chem. 271: 18582-18587, 1996. PubMed: 8702508

Bruant SS, et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J. Biol. Chem. 271: 5195-5199, 1996. PubMed: 8617802

Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793

Kawabe J, et al. Soluble adenylyl cyclase from Spodoptera frugiperda (Sf9) cells. J. Biol. Chem. 271: 20132-20137, 1996. PubMed: 8702736

Yasuda H, et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595, 1996. PubMed: 8702509

Sf9 was cloned by G. E. Smith and C. L. Cherry from the parent line, IPLB-SF 21 AE, which was derived from pupal ovarian tissue of the fall armyworm, Spodoptera frugiperda, by Vaughn, et al., in 1977.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Vaughn JL, et al. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13: 213-217, 1977. PubMed: 68913

Zhang PF, et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-213, 1994. PubMed: 7811453

Smith GE, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985. PubMed: 3878519

Hall MR, Gibson W. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separately cloned and coexpressed in eukaryotic cells. J. Virol. 70: 5395-5404, 1996. PubMed: 8764050

Bakker A, et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J. Virol. 70: 5511-5518, 1996. PubMed: 8764063

Gibson W, et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J. Virol. 70: 7454-7461, 1996. PubMed: 8892863

Filtz TM, et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J. Biol. Chem. 49: 31121-31126, 1996. PubMed: 8940109

Lindorfer MA, et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J. Biol. Chem. 271: 18582-18587, 1996. PubMed: 8702508

Bruant SS, et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J. Biol. Chem. 271: 5195-5199, 1996. PubMed: 8617802

Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793

Kawabe J, et al. Soluble adenylyl cyclase from Spodoptera frugiperda (Sf9) cells. J. Biol. Chem. 271: 20132-20137, 1996. PubMed: 8702736

Yasuda H, et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595, 1996. PubMed: 8702509

Sf9 was cloned by G. E. Smith and C. L. Cherry from the parent line, IPLB-SF 21 AE, which was derived from pupal ovarian tissue of the fall armyworm, Spodoptera frugiperda, by Vaughn, et al., in 1977.