AsPC-1 (ATCC® CRL-1682)

Organism: Homo sapiens, human  /  Tissue: pancreas; derived from metastatic site: ascites  /  Disease: adenocarcinoma

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Organism Homo sapiens, human
Tissue pancreas; derived from metastatic site: ascites
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 62 years
Gender female
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
Clinical Data
62 years
Caucasian
female

Genes Expressed
carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
Cellular Products
carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
    Medium Renewal: 2 to 3 times per week
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    STR Profile
    Amelogenin: X
    CSF1PO: 10,13
    D13S317: 9,12
    D16S539: 11
    D5S818: 12
    D7S820: 12,13
    THO1: 7,9.3
    TPOX: 8,10
    vWA: 17
    Name of Depositor MH Tan
    References

    Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348

    Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528

    Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226

    Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

    Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565

    Cross References

    Nucleotide (GenBank) : AA640215 01 subtracted 3' EST library Homo sapiens cDNA clone U32, mRNA sequence.

    Nucleotide (GenBank) : AA640216 02 subtracted 3' EST library Homo sapiens cDNA clone U30, mRNA sequence.

    Nucleotide (GenBank) : AA640217 03 subtracted 3' EST library Homo sapiens cDNA clone D32, mRNA sequence.

    Nucleotide (GenBank) : AA640218 04 subtracted 3' EST library Homo sapiens cDNA clone D42, mRNA sequence.

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    Other Documentation
    References

    Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348

    Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528

    Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226

    Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

    Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565