Human genomic DNA (PZ-HPV-7) (ATCC® CRL-2221D)

Organism: Homo sapiens, human  / 

Permits and Restrictions

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Product Format frozen Approx. 10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level 2
Introduction

Cell Line Description:

Epithelial cells cultured from normal tissue of the peripheral zone of the prostate from a 70-year-old Caucasian male were transfected with HPV-18 DNA. Incorporation of HPV-18 DNA was confirmed by polymerase chain reaction; specific amplification of a 160-base pair fragment of the HPV-18 E6-transforming region was noted. Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 oncoprotein of HPV. The cells are negative for prostate specific antigen (PSA).

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Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation