CHP 3 (M.W.) (ATCC® CCL-132)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 6 years
Gender male
Ethnicity Black
Applications
His older sibling (W.W.) lacks the same enzyme but exhibited no clinical symptoms of galactosemia.
This fibroblast cell line was established by W.J. Mellman from a skin biopsy of a 6-year-old Black male with galactosemia.
This child demonstrated a classic galactosemia picture in infancy and lacks galactose-1-phosphate uridyl transferase activity in his circulating red cells.
Karyotype normal human male; stable
Derivation
This fibroblast cell line was established by W.J. Mellman from a skin biopsy of a 6-year-old Black male with galactosemia. This child demonstrated a classic galactosemia picture in infancy and lacks galactose-1-phosphate uridyl transferase activity in his circulating red cells. His older sibling (W.W.) lacks the same enzyme but exhibited no clinical symptoms of galactosemia. [Cell line CHP4, ATCC CCL-133]
Clinical Data
This child demonstrated a classic galactosemia picture in infancy and lacks galactose-1-phosphate uridyl transferase activity in his circulating red cells.
This fibroblast cell line was established by W.J. Mellman from a skin biopsy of a 6-year-old Black male with galactosemia.
male
Black
6 years
Comments
This fibroblast cell line was established by W.J. Mellman from a skin biopsy of a 6-year-old Black male with galactosemia. This child demonstrated a classic galactosemia picture in infancy and lacks galactose-1-phosphate uridyl transferase activity in his circulating red cells. His older sibling (W.W.) lacks the same enzyme but exhibited no clinical symptoms of galactosemia. [Cell line CHP4, ATCC CCL-133]
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 1 to 2 times per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37C.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Isoenzymes
G6PD, A
Name of Depositor WJ Mellman
References

. . J. Pediatr. 68: 551, 1966.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . J. Pediatr. 68: 551, 1966.