CHP 4 (W.W.) (ATCC® CCL-133)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 8 years
Gender male
Ethnicity Black
Applications
Metabolic studies appear to place him in a subgroup of atypical galactosemic patients as described by Segal et al.
This child had never exhibited any symptoms of galactosemia although he lacked galactose-1-phosphate uridyl transferase activity in his circulating red cells.
This fibroblast cell line was established by W.J. Mellman from a skin biopsy of an 8-year-old Black male with asymptomatic galactosemia.
His younger sibling, (M.W.) demonstrated the classic galactosemic picture in infancy [Cell line CHP3, ATCC CCL-132].
Karyotype normal human male; diploid; stable
Derivation
This fibroblast cell line was established by W.J. Mellman from a skin biopsy of an 8-year-old Black male with asymptomatic galactosemia. This child had never exhibited any symptoms of galactosemia although he lacked galactose-1-phosphate uridyl transferase activity in his circulating red cells. Metabolic studies appear to place him in a subgroup of atypical galactosemic patients as described by Segal et al. His younger sibling, (M.W.) demonstrated the classic galactosemic picture in infancy [Cell line CHP3, ATCC CCL-132].
Clinical Data
This child had never exhibited any symptoms of galactosemia although he lacked galactose-1-phosphate uridyl transferase activity in his circulating red cells.
This fibroblast cell line was established by W.J. Mellman from a skin biopsy of an 8-year-old Black male with asymptomatic galactosemia.
Metabolic studies appear to place him in a subgroup of atypical galactosemic patients as described by Segal et al.
male
Black
8 years
Comments
This fibroblast cell line was established by W.J. Mellman from a skin biopsy of an 8-year-old Black male with asymptomatic galactosemia. This child had never exhibited any symptoms of galactosemia although he lacked galactose-1-phosphate uridyl transferase activity in his circulating red cells. Metabolic studies appear to place him in a subgroup of atypical galactosemic patients as described by Segal et al. His younger sibling, (M.W.) demonstrated the classic galactosemic picture in infancy [Cell line CHP3, ATCC CCL-132].
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37C.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Isoenzymes
G6PD, B
Name of Depositor WJ Mellman
References

. . J. Pediatr. 68: 551, 1966.

Segal S, et al. The metabolism of galactose by patients with congenital galactosema. Am. J. Med. 38: 62-70, 1965. PubMed: 14247292

Basic Documentation
References

. . J. Pediatr. 68: 551, 1966.

Segal S, et al. The metabolism of galactose by patients with congenital galactosema. Am. J. Med. 38: 62-70, 1965. PubMed: 14247292