WS1 (ATCC® CRL-1502)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: skin  /  Disease: normal

Organism Homo sapiens, human
Tissue
skin
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 12 week gestation
Gender female
Ethnicity Black
Storage Conditions liqudi nitrogen vapor phase
Derivation
WS1 was derived from normal human skin by RJ Hay.
Clinical Data
12 week gestation
Black
female
Comments
WS1 cells have a doubling potential of 67 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liqudi nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 10,11
D5S818: 13
D7S820: 9,10
THO1: 8,10
TPOX: 8,9
vWA: 17,18
Population Doubling Capacity WS1 cells have a doubling potential of 67 population doublings.
Name of Depositor RJ Hay
References

Huang LC, et al. Sensitivity and selectivity of the DNA damage sensor responsible for activating p53-dependent G1 arrest. Proc. Natl. Acad. Sci. USA 93: 4827-4832, 1996. PubMed: 8643488

Corfield VA, Hay RJ. Effects of cystine or glutamine restriction on human diploid fibroblasts in culture. In Vitro 14: 787-794, 1978. PubMed: 721103

Cross References

Nucleotide (GenBank) : U19758 Human chromosome 7 beta-actin region clone pCC2Dee.

Nucleotide (GenBank) : U19757 Human chromosome 7 beta-actin region clone pCC10Jhb.

Basic Documentation
References

Huang LC, et al. Sensitivity and selectivity of the DNA damage sensor responsible for activating p53-dependent G1 arrest. Proc. Natl. Acad. Sci. USA 93: 4827-4832, 1996. PubMed: 8643488

Corfield VA, Hay RJ. Effects of cystine or glutamine restriction on human diploid fibroblasts in culture. In Vitro 14: 787-794, 1978. PubMed: 721103