CCD-43Sk (ATCC® CRL-1509)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 1 week
Gender male
Ethnicity Caucasian
Applications
The line was derived from normal skin of a child that died of necrotizing enterocolitis.
Derivation
The line was derived from normal skin of a child that died of necrotizing enterocolitis.
Clinical Data
The line was derived from normal skin of a child that died of necrotizing enterocolitis.
male
Caucasian
1 week
Comments
The line was derived from normal skin of a child that died of necrotizing enterocolitis.
The line has undergone approximately 21 population doublings in culture.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.