CCD-1093Sk (ATCC® CRL-2115)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 27 years
Gender male
Ethnicity Caucasian
Applications
The line was established from normal skin of the forearm at amputation.
Derivation
The line was established from normal skin of the forearm at amputation.
Clinical Data
The patient suffered from nonfunctional atrophy and fibrosis.
male
Caucasian
27 years
Comments
The line was established from normal skin of the forearm at amputation.
The patient suffered from nonfunctional atrophy and fibrosis.
The cells senesce after approximately 42 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cryopreservation
Culture medium, 95%; DMSO, 5%