TE 354.T (ATCC® CRL-7762)

Organism: Homo sapiens, human  /  Tissue: skin  /  Disease: basal cell carcinoma

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Organism Homo sapiens, human
Tissue skin
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease basal cell carcinoma
Gender female
Storage Conditions liquid nitrogen vapor phase
Images
Clinical Data
female

Comments

The patient was sister of donor for ATCC CRL-7714.

CRL-7762 may grow very slowly.  The cells may recover from cryopreservation slowly and may take as long as 10-14 days after thaw before a subculture can be performed.

Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Note: CRL 7762 may grow very slowly. The cells may recover from cryopreservation slowly and may take as long as 10-14 days after thaw before a subculture can be performed


Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,14
D13S317: 11,14
D16S539: 12
D5S818: 10,13
D7S820: 10
THO1: 6
TPOX: 8,12
vWA: 14,17
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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