F32 VIII C4 (ATCC® CRL-1653)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, suspension
Biosafety Level 1
Strain BALB/c
Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Mice were immunized with purified hog renin.
Spleen cells were fused with Sp2/0 myeloma cells.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody; against hog renin,H-2d
Comments
Mice were immunized with purified hog renin.
Spleen cells were fused with Sp2/0 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Subculturing
Protocol: Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Medium Renewal: 2 to 3 times per week
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Isotype IgG1
Name of Depositor LT Skeggs
References

Dorer FE, et al. Purification of hog kidney remin with immobilized monoclonal antirenin. Hypertension 6: 374-382, 1984. PubMed: 6376344

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Dorer FE, et al. Purification of hog kidney remin with immobilized monoclonal antirenin. Hypertension 6: 374-382, 1984. PubMed: 6376344