SW 1990 [SW-1990, SW1990] (ATCC® CRL-2172)

Organism: Homo sapiens, human  /  Tissue: pancreas; Derived from metastatic site: spleen  /  Disease: adenocarcinoma

Permits and Restrictions

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Organism Homo sapiens, human
Tissue pancreas; Derived from metastatic site: spleen
Product Format frozen
Morphology Epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 56 years
Gender Male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype near triploid; range = 67 to 75
Images
Derivation
The SW 1990 line was established in 1978 from a spleen metastasis of a grade II pancreatic adenocarcinoma derived from the exocrine pancreas.

Clinical Data
56 years
Caucasian
male
Antigen Expression
Blood Type A; Rh +
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Comments
The cells have a reported plating efficiency of 29%.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week


Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 100%
STR Profile
D5S818: 12, 13
D13S317: 8, 12
D7S820: 9, 10
D16S539: 13
vWA: 17
THO1: 9.3
Amelogenin: X
TPOX: 8, 9
CSF1PO: 10, 12

Population Doubling Time 64 hrs
Name of Depositor W McCombs
Year of Origin 1978
References

Kyriazis AP, et al. Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse. Cancer Res. 43: 4393-4401, 1983. PubMed: 6871872

Basic Documentation
Other Documentation
Restrictions

These cells are distributed for research purposes only. The Scott and White Clinic releases the line subject to the following: 1) The cells or products derived from them must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. Commercial interests are the exclusive property of the Scott and White Clinic. 2) Any proposed commercial use of these cells or products produced by them must first be negotiated with the Scott and White Clinic, 2401 S. 31 Street, Temple, Texas 76508. Telephone (817) 774-2432

References

Kyriazis AP, et al. Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse. Cancer Res. 43: 4393-4401, 1983. PubMed: 6871872