MG38 (ATCC® CRL-2640)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells.
The antibody reacts with DEC-205 in tissue sections from both humans and rhesus macaque monkeys.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
DEC-205-/- knock-out mice were immunized with purified protein from human DEC-205 cells.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the human DEC-205 antigen (human dendritic cell antigen)
Comments
DEC-205-/- knock-out mice were immunized with purified protein from human DEC-205 cells.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells.
In tissue sections, MG38 identifies DEC-205 on thymic cortical epithelium and dendritic cells in the thymic medulla and tonsillar T cell areas.
The antibody reacts with DEC-205 in tissue sections from both humans and rhesus macaque monkeys.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Culture medium with an additional 5% fetal bovine serum, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG2b
Name of Depositor RM Steinman
References

Guo M, et al. A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum. Immunol. 61: 729-738, 2000. PubMed: 10980384

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Guo M, et al. A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum. Immunol. 61: 729-738, 2000. PubMed: 10980384