I1-Hybridoma (ATCC® CRL-2700)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Vesicular Stomatitis

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Vesicular Stomatitis
Age adult
Applications
The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
Tumorigenic Yes
Effects
Yes, intraperitoneal injection into BALB/c or (BALB/c X BALB.B)F1 mice
Comments
Animals were immunized with vesicular stomatitis virus, Indiana Serotype (VSV-Ind.).
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind.).
Complete Growth Medium Minimum essential medium (Eagle) with 2.0 mM L-glutamine and Hanks' BSS adjusted to contain 0.35 g/L sodium bicarbonate 80%; fetal bovine serum, 20%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 45% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Growth Conditions: This medium is formulated for use in a free gas exchange with atmospheric air. A 5% CO2 in air atmosphere is detrimental to cells when using this medium for culturing.
Isotype mouse IgG2a kappa
Name of Depositor IS Novella, DS Lyles
References

Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982. PubMed: 6180550

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982. PubMed: 6180550