20H11 (ATCC® CRL-9300)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte; Epstein-Barr virus (EBV) transforme  / 

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Organism Homo sapiens, human
Cell Type B lymphocyte; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Applications
The antibody reacts with P aeruginosa Fisher immunotypes 2, 6 and 7.
The antibody cross-reacts extensively with clinical isolates of P. aeruginosa bearng type b flagella.
Clinical Data
Peripheral blood B cells from a patient immunized with high molecular weight polysaccharide from P. aeruginosa were transformed with Epstein-Barr virus.
Genes Expressed
immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa (flagella type b)
Cellular Products
immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa (flagella type b)
Comments
Peripheral blood B cells from a patient immunized with high molecular weight polysaccharide from P. aeruginosa were transformed with Epstein-Barr virus.
The antibody reacts with P aeruginosa Fisher immunotypes 2, 6 and 7.
The antibody cross-reacts extensively with clinical isolates of P. aeruginosa bearng type b flagella.
Complete Growth Medium Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 85%; heat-inactivated fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Population Doubling Time 24 to 36 hrs
Name of Depositor Genetic Systems Corp.
References

Rosok MJ, Lostrom ME. Monoclonal antibodies to Pseudomonas aeruginosa flagella. US Patent 4,834,976 dated May 30 1989

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rosok MJ, Lostrom ME. Monoclonal antibodies to Pseudomonas aeruginosa flagella. US Patent 4,834,976 dated May 30 1989