MD (ATCC® CRL-9850)

Organism: Homo sapiens, human  /  Cell Type: monocyte/macrophage  /  Tissue: spleen  / 

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Organism Homo sapiens, human
Tissue
spleen
Cell Type monocyte/macrophage
Product Format frozen
Morphology monocyte/macrophage
Culture Properties loosely adherent, multicell aggregates
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions liquid nitrogen vapor phase
Derivation
The MD cell line was established from cryopreserved human spleen cells.
Comments Epstein-Barr virus has been detected in this cell line.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 X 105 viable cells/mL. A maximum of 1 to 2 X 106 is obtainable.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11,15
D5S818: 11,13
D7S820: 10,11
THO1: 8
TPOX: 8,11
vWA: 16,17
Name of Depositor E.I. du Pont de Nemours & Co.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995