MD (ATCC® CRL-9850)

Organism: Homo sapiens, human  /  Cell Type: monocyte/macrophage  /  Tissue: spleen  / 

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Organism Homo sapiens, human
Tissue
spleen
Cell Type monocyte/macrophage
Product Format frozen
Morphology monocyte/macrophage
Culture Properties loosely adherent, multicell aggregates
Biosafety Level 1
Storage Conditions liquid nitrogen vapor phase
Derivation
The MD cell line was established from cryopreserved human spleen cells.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 X 105 viable cells/mL. A maximum of 1 to 2 X 106 is obtainable.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11,15
D5S818: 11,13
D7S820: 10,11
THO1: 8
TPOX: 8,11
vWA: 16,17
Name of Depositor E.I. du Pont de Nemours & Co.
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995