QCRL-3 (ATCC® HB-11766)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Carcinoma

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Carcinoma
Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with MRP (multidrug resistance protein).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a multidrug resistance protein (MRP)
Cellular Products
immunoglobulin; monoclonal antibody; against a multidrug resistance protein (MRP)
Comments
Animals were immunized with the multidrug resistant, small cell lung carcinoma, cell line H69AR (ATCC CRL-11351).
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody reacts with MRP (multidrug resistance protein). MRP is a 190,000 integral membrane phosphoglycoprotein that is overexpressed in some drug-selected resistant cell lines and has been shown to cause multidrug resistance in transfected cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG2a kappa
Name of Depositor Queen's University at Kingston
References

Deeley RG, Cole SP. Antibodies to a multidrug resistance protein. US Patent 6,063,621 dated May 16 2000

Hipfner DR, et al. Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies. Cancer Res. 54: 5788-5792, 1994. PubMed: 7954400

Basic Documentation
References

Deeley RG, Cole SP. Antibodies to a multidrug resistance protein. US Patent 6,063,621 dated May 16 2000

Hipfner DR, et al. Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies. Cancer Res. 54: 5788-5792, 1994. PubMed: 7954400