R4-6A2 (ATCC® HB-170)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: Hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast
Culture Properties Suspension
Biosafety Level 1
Applications

The antibody neutralizes the ability of lymphokine preparations to induce tumoricidal activity in murine macrophages. 

The antibody neutralizes the antiviral effect of gamma interferon but does not affect the activities of murine alpha and beta interferon or rat or human gamma interferon.

30 ng of R4-6A2 antibody neutralizes the antiviral activity of 10 units of murine gamma interferon.

Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with partially purified murine gamma interferon. 

Spleen cells were fused with P3U-1 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against mouse gamma interferon
Tumorigenic Yes
Effects

Forms ascites in nude mice.

Comments

The antibody neutralizes the ability of lymphokine preparations to induce tumoricidal activity in murine macrophages. 

The antibody neutralizes the antiviral effect of gamma interferon but does not affect the activities of murine alpha and beta interferon or rat or human gamma interferon.

30 ng of R4-6A2 antibody neutralizes the antiviral activity of 10 units of murine gamma interferon.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 viable cells/mL. Maintain cultures at a cell concentration between 6 x 104 and 6 x 105 cells/mL. Do not allow the cell concentration to reach 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete culture medium, 92.5%; DMSO, 7.5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Note: Do not allow the cell concentration to reach 1 x 106 cells/mL.
Isotype IgG1
Name of Depositor GL Spitalny
References

Spitalny GL, Havell EA. Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. J. Exp. Med. 159: 1560-1565, 1984. PubMed: 6425450

Tay CH, Welsh RM. Distinct organ-dependent mechanisms for the control of murine cytomegalovirus infection by natural killer cells. J. Virol. 71: 267-275, 1997. PubMed: 8985346

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

Skeen MJ, et al. Regulation of murine macrophage IL-12 production. J. Immunol. 156: 1196-1206, 1996. PubMed: 8557998

Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Spitalny GL, Havell EA. Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. J. Exp. Med. 159: 1560-1565, 1984. PubMed: 6425450

Tay CH, Welsh RM. Distinct organ-dependent mechanisms for the control of murine cytomegalovirus infection by natural killer cells. J. Virol. 71: 267-275, 1997. PubMed: 8985346

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

Skeen MJ, et al. Regulation of murine macrophage IL-12 production. J. Immunol. 156: 1196-1206, 1996. PubMed: 8557998

Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm