c9189 [9189] (ATCC® 70888)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: MV Olson, L Riles

Designations c9189 [9189]
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors MV Olson, L Riles
Applications
The ATCC encourages users of this material to communicate their results to us.
Vector
Construct size (kb): 39.5
Insert
DNA: genomic

Insert information:

Insert size (kb): 33.1

Gene : HMR (mating-type cassette)

DNA : genomic DNA

Contains complete coding sequence : Unknown

Source : Saccharomyces cerevisiae AB972 (ATCC# 76269)

Chromosome : III

                Localization : III R, 97 cM from CEN

Insert ends : MboI

Insert lengths(kb): 33.09999847412109
Gene product: mating-type cassette [HMR]
Insert Size (kb) 33.100
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
component of:ATCC 77284
Biosafety Level 1
Shipping Information Frozen glycerol stock of E. coli containing the cosmid

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.6, 4.4, 4.1, 2.5, 2.3 (doublet), 2.1, 1.9 (doublet), 1.7, 1.6, 1.3 (doublet), 0.9 (doublet), 0.8, 0.6 (triplet), 0.5 (doublet), 0.35.
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.267, 0.937, 0.790, 0.485, 2.085, 1.906, 1.533, 0.429, 1.983, 1.295, 4.392, 4.113, 0.891, 0.609, 6.113, 2.390, 0.486, 2.593, 2.368, 1.630, 0.606, 0.571.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Overlaps clones with ATCC number(s): 70206, 70863, 70896.
NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

Wicksteed BL, et al. A physical comparison of chromosome III in six strains of Saccharomyces cerevisiae. Yeast 10: 39-57, 1994. PubMed: 8203151

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