c9871 (ATCC® 71236)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: R Hyman

Designations c9871
GenBank Number

U18530

U18779

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors R Hyman
Vector
Construct size (kb): 44.43000030517578
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: CUP5
Gene name: vacuolar ATP synthase, 16 kDa proteolipid subunit;
dicyclohexylcarbodiimide binding subunit
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: RIP1
Gene name: Rieske iron-sulfur protein of the mitochondrial cytochrome
bc1 complex
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: URA3
Gene name: orotidine-5'-phosphate decarboxylase
Contains complete coding sequence?: Y
Chromosome: V; Localization: V L, 7 cM from CEN
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: RPR1
Gene name: RNA component of nuclear RNase P
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: MMS21
Contains complete coding sequence?: Y
Chromosome: V
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 38.0
Cross references: DNA Seq. Acc.: U18530
Nucleotides ? of the insert correspond to
nucleotides 1-38086 of U18530.
DNA Seq. Acc.: U18779
Nucleotides ? of the insert correspond to
nucleotides 31670-36872 of U18779.
Insert lengths(kb): 38.0
Gene product: Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex [CUP5]
Insert Size (kb) 38.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen, uncharacterized
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Product Sheet
Product Sheet
Product Sheet