c9871 (ATCC® 71236)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: R Hyman

Permits and Restrictions

View Permits

Designations c9871
GenBank Number

U18530

U18779

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors R Hyman
Vector
Construct size (kb): 44.43000030517578
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: CUP5
Gene name: vacuolar ATP synthase, 16 kDa proteolipid subunit;
dicyclohexylcarbodiimide binding subunit
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: RIP1
Gene name: Rieske iron-sulfur protein of the mitochondrial cytochrome
bc1 complex
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: URA3
Gene name: orotidine-5'-phosphate decarboxylase
Contains complete coding sequence?: Y
Chromosome: V; Localization: V L, 7 cM from CEN
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: RPR1
Gene name: RNA component of nuclear RNase P
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: MMS21
Contains complete coding sequence?: Y
Chromosome: V
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 38.0
Cross references: DNA Seq. Acc.: U18530
Nucleotides ? of the insert correspond to
nucleotides 1-38086 of U18530.
DNA Seq. Acc.: U18779
Nucleotides ? of the insert correspond to
nucleotides 31670-36872 of U18779.
Insert lengths(kb): 38.0
Gene product: Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex [CUP5]
Insert Size (kb) 38.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen, uncharacterized
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
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