Primary Cell Culture Reagents and Media

  • Renal Epithelial Cell Basal Medium (ATCC® PCS-400-030)

    ATCC® Number: PCS-400-030
    Quantity: 485 mL
    Storage: 2 to 8°C, protect from light
    Applications: Renal Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. Renal Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of renal epithelial cells (e.g., Primary Renal Proximal Tubule Epithelial Cells, Normal, Human, ATCC PCS-400-010; Primary Renal Cortical Epithelial Cells, Normal, Human, ATCC PCS-400-011; Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC PCS-400-012), Renal Cell Basal Medium must be supplemented with the Renal Epithelial Cell Growth Kit (ATCC PCS-400-040).

    Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution  (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

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  • Vascular Cell Basal Medium (ATCC® PCS-100-030)

    ATCC® Number: PCS-100-030
    Quantity: 475 mL
    Storage: 2°C to 8°C, protect from light
    Applications: Vascular Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system.

    This system is designed to support cells derived from normal human large vessels such as human umbilical vein endothelial cells, aortic endothelial cells or aortic smooth muscle cells, as well as microvascular endothelial cells. Vascular Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of cells derived from the vascular system, Vascular Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit.

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  • Dermal Cell Basal Medium (ATCC® PCS-200-030)

    ATCC® Number: PCS-200-030
    Quantity: 485 ml
    Storage: 2 to 8°C, protect from light
    Applications: Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions™ system.

    This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. 

    To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin, ATCC PCS-200-010), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit (ATCC PCS-200-040). The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation.

    To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCC PCS-200-012 or Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit (ATCC PCS-200-041) or the Adult Melanocyte Growth Kit (ATCC PCS-200-042).

    Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

     

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  • Airway Epithelial Cell Basal Medium (ATCC® PCS-300-030)

    ATCC® Number: PCS-300-030
    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Airway Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This serum-free system is designed to support airway epithelial cells derived from normal human lung. Airway Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of airway epithelial cells, Airway Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of airway epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. 

    1. For airway epithelial cells derived from bronchial/tracheal tissue (e.g., Primary Bronchial/Tracheal Epithelial Cells, Normal, Human, ATCC PCS-300-010), supplement Airway Epithelial Cell Basal Medium with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040).
    2. For small airway epithelial cells (e.g., Primary Small Airway Epithelial Cells, Normal, Human, ATCC PCS-301-010), supplement Airway Epithelial Cell Basal Medium with the Small Airway Epithelial Cell Growth Kit (ATCC PCS-301-040).
    3. Optional media supplements:
      1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
      2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
      3. Phenol Red (ATCC PCS-999-001)

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