145-2C11 (ATCC® CRL-1975)

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)  /  Cell Type: Hybridoma: B lymphocyte  /  Tissue: Spleen, blood  / 

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Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Tissue Spleen, blood
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast-like
Culture Properties Suspension
Biosafety Level 1
Applications

It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor. The antibody reacts with the murine T cell receptor (CD3 - T3) complex.

The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.

The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb). Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD3
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype IgG , IgG
Name of Depositor JA Bluestone
References

Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524

Kayagaki N, et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524

Kayagaki N, et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079