1G3 (ATCC® CRL-2434)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties mixed: adherent and suspension
Biosafety Level 1
Applications
This antibody is used in studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG. RefRaghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418
Storage Conditions liquid nitrogen vapor phase
Derivation

The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992. Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).

Genes Expressed
immunoglobulin; monoclonal antibody; against FcRn heavy chain heterodimers
Cellular Products
immunoglobulin; monoclonal antibody; against FcRn heavy chain heterodimers
Comments


FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. 

The antibody recognizes FcRn heavy chain heterodimers. 

FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules. 

The IG3 (ATCC CRL-2434) antibody recognizes two lipid linked forms of FcRn. 

These forms are truncated FcRn heavy chain paired with beta 2-microgloublin-DAF (FcRn beta-2 microglobulin DAF) and lipid-linked FcRn paired with unaltered beta 2-microgloublin (FcRn-DAF/beta 2-microgloublin). 

It also recognizes the FcRn-DAF construct. 

The 2G3 (ATCC CRL-2435) antibody fails to recognize the FcRn heterodimer form in which the heavy chain is attached to the lipid anchor (FcRn-DAF/beta 2-microgloublin) and it also fails to recognize the FcRn-DAF construct. 

Unlike 4C9 (ATCC CRL-2437), the monoclonal antibodies IG3 (ATCC CRL-2434) and 2G3 (ATCC CRL-2435) do not recognize rat beta 2-microglobulin alone or complexed with FcRn heavy chains. 

Complete Growth Medium HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).
Subculturing

Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 x 105 viable cells/mL.

Subcultivation Ratio: A subcultivation ratio of 1:5 every 3 to 4 day is recommended
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).

Cryopreservation
HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Isotype IgG1
Name of Depositor PJ Bjorkman
Year of Origin 1992
References

Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418

Basic Documentation
References

Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418