4B2 (ATCC® HB-196)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications

The antibody reacts with all types of human leukocytes, but not with non-leukocytes.

The antibody binds to protein A.

Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with human T cells.

Spleen cells were fused with P3U1 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)
Tumorigenic Yes, in irradiated pristane primed (BALB/c X DBA/2)F1 mice.
Comments

The antibody reacts with all types of human leukocytes, but not with non-leukocytes.

The antibody binds to protein A.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.

Culture Conditions Temperature: 37°C
Isotype IgG2a
Name of Depositor RM Steinman
References

. Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ. Press; 1987.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ. Press; 1987.