4B2 (ATCC® HB-196)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications

The antibody reacts with all types of human leukocytes, but not with non-leukocytes.

The antibody binds to protein A.

Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with human T cells.

Spleen cells were fused with P3U1 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)
Cellular Products
immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)
Tumorigenic Yes, in irradiated pristane primed (BALB/c X DBA/2)F1 mice.
Comments

The antibody reacts with all types of human leukocytes, but not with non-leukocytes.

The antibody binds to protein A.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.

Culture Conditions Temperature: 37°C
Isotype IgG2a
Name of Depositor RM Steinman
References

. Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ. Press; 1987.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Basic Documentation
References

. Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ. Press; 1987.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.