1D3 (ATCC® HB-305)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
It is useful flow cytometric analysis of total mouse B cells and for studies of signal transduction involving CD19.
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was produced by fusing NS-1 myeloma cells with spleen cells from Lewis rats that had been immunized with transfected K562 cells expressing the extracellular domain of murine CD19.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD19
Comments

The hybridoma cell line 1D3 secretes a rat monoclonal antibody that reacts with the mouse pan-B cell marker CD19.

The antibody binds to mouse CD19 with high affinity, but does not react with human CD19.

Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium. Establish new cultures at 2 x 10viable cells/mL and maintain at between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Isotype IgG2a; kappa light chain
Name of Depositor DT Fearon, I Krop, MS Schlissel, AR de Fougerolles
References

Krop I, et al. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur. J. Immunol. 26: 238-242, 1996. PubMed: 8566073

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Krop I, et al. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur. J. Immunol. 26: 238-242, 1996. PubMed: 8566073