Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 43598)

Strain Designations: 1470  /  Type Strain: no  /  Biosafety Level: 2

Strain Designations 1470
Application
Enteric Research
Isolation
Human feces, asymptomatic neonate, Belgium
Type Strain no
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Antigenic Properties Serogroup F RefDelmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688, RefDelmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype Toxinotype: VIII RefRupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Type Strain no
Antigenic Properties Serogroup F RefDelmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688, RefDelmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463
Comments
Presence of tcdB gene confirmed by PCR.
Binary toxin gene cdtB not amplified by PCR
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Toxinotype VIII
Ribotype 017
Genotype Toxinotype: VIII RefRupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Name of Depositor M Delmee
Isolation
Human feces, asymptomatic neonate, Belgium
References

Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688

Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688

Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688

Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999