pCFM536 plasmid in E. coli (ATCC® 39934)

Applications: Expression vector  /  Depositors: Amgen, Inc., F Lin, Amgen, Inc.

Designations pCFM536 plasmid in E. coli
Depositors Amgen, Inc., F Lin, Amgen, Inc.
Biosafety Level 1
Host
Distribution host: Escherichia coli JM103/pMW1
Vector Information
Vector information for pCRM536:

Vector Type: plasmid

Vector size (kb): 4.6

Markers: ampR

Promoter: lambda PL

Replicon: R1

Vector information for pMW1 (ATCC 39933):

Vector Type: plasmid

Vector size (kb): 12.0

Markers: kanR, tetR

Replicon: pSH1

Repressor gene: cI857

Applications
Expression vector
Comments
Ampicillin is the selection marker for pCFM526, kanamycin for pMW1.
Temperature sensitive, grow at 28°C.
This is an expression plasmid containing a lambdaPL promoter, a multiple cloning sequence, and a lambda Toop terminator.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 28°C
References

Lin FK. DNA sequences encoding erythropoietin. US Patent 4,703,008 dated Oct 27 1987

Morris CF. DNA plasmids. US Patent 4,710,473 dated Dec 1 1987

Shipped frozen
Shipping Information Frozen glycerol stock of Escherichia coli containing the plasmid.
Product Sheet
Product Sheet
Product Sheet
Attachments Product Information Sheet