pACHRBcDNA [ACh receptor beta cDNA] (ATCC® 63104)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: JP Merlie

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Designations pACHRBcDNA [ACh receptor beta cDNA]
GenBank Number

M14537

Species Mus musculus, mouse
Depositors JP Merlie
Vector
Construct size (kb): 5.0
Insert
DNA: cDNA
Insert lengths(kb): 2.075000047683716
Tissue: muscle BC3H-1 cell line
Gene product: cholinergic receptor, nicotinic, beta polypeptide 2( cholinergic receptor, nicotinic, beta subunit 2, acetylcholine receptor beta 2 neural) [Acrb]
Insert Size (kb) 2.075
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
The insert contains the following restriction sites (nt position according to published sequence): SacII--68; PvuII--450; PstI--455, 1538; BamHI--556; BglII--1044, 1858, 1918; BstXI--1399.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; BamHI--3.4, 1.7; HindIII--5.0; PstI--3.6, 1.1, 0.6; BglII--4.2, 0.9.
Contains the complete coding sequence (nt 2-1507).
Detects a mRNA of 2.4 kb.
Oriented such that transcription from the SP6 promoter gives the sense strand.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Buonanno A, et al. A universal oliglonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit. J. Biol. Chem. 261: 16451-16458, 1986. PubMed: 3782129

Heidmann O, et al. Chromosomal localization of muscle nicotinic acetylcholine receptor genes in the mouse. Science 234: 866-868, 1986. PubMed: 3022377

John P Merlie, personal communication

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