pACHRBcDNA [ACh receptor beta cDNA] (ATCC® 63104)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: JP Merlie

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Designations pACHRBcDNA [ACh receptor beta cDNA]
GenBank Number

M14537

Species Mus musculus, mouse
Depositors JP Merlie
Vector
Construct size (kb): 5.0
Insert
DNA: cDNA
Insert lengths(kb): 2.075000047683716
Tissue: muscle BC3H-1 cell line
Gene product: cholinergic receptor, nicotinic, beta polypeptide 2( cholinergic receptor, nicotinic, beta subunit 2, acetylcholine receptor beta 2 neural) [Acrb]
Insert Size (kb) 2.075
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
The insert contains the following restriction sites (nt position according to published sequence): SacII--68; PvuII--450; PstI--455, 1538; BamHI--556; BglII--1044, 1858, 1918; BstXI--1399.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; BamHI--3.4, 1.7; HindIII--5.0; PstI--3.6, 1.1, 0.6; BglII--4.2, 0.9.
Contains the complete coding sequence (nt 2-1507).
Detects a mRNA of 2.4 kb.
Oriented such that transcription from the SP6 promoter gives the sense strand.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Buonanno A, et al. A universal oliglonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit. J. Biol. Chem. 261: 16451-16458, 1986. PubMed: 3782129

Heidmann O, et al. Chromosomal localization of muscle nicotinic acetylcholine receptor genes in the mouse. Science 234: 866-868, 1986. PubMed: 3022377

John P Merlie, personal communication

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