pET-His (ATCC® 87036)

Applications: expression vector  /  Depositors: T Hai

Permits and Restrictions

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Designations pET-His
Depositors T Hai
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

E. coli HB101
Vector Information
Size (kb): 4.636
Vector: pET-His (plasmid)
Promoters: Promoter T7 (phi10)
Construction: pET3a
Construct size (kb): 4.636
Features: marker(s): ampR
promoter: T7 (phi10)
replicon: pMB1
MCS: NdeI...BamHI
terminator: phi10
expression vector
Restriction digests of the clone give the following sizes (kb): EcoRI--4.6; BamHI--4.6; BglI--2.5, 2.1, 0.25.
The plasmid contains the following restriction sites (kb from nt 1): BamHI--4.12; BglI--0.87, 3.19, 3.42; BglII--3.99; EcoRI--4.63; HindIII--4.60; NcoI--4.12; NdeI--4.09; PstI--0.75; PvuI--0.62; PvuII--2.29; SalI--3.71; XbaI--4.05; XhoI--4.11.
Digestion with NcoI, BamHI or XhoI and subsequent filling of ends, allows cloning of blunt-ended fragments with a +0, +1 (C) or +2 (GA) nucleotide reading frame, respectively.
Fusion proteins can be purified using a nickel-chelating column, even under denaturing conditions.
Constructed from pET3a by insertion of a double stranded oligonucleotide linker between the NdeI and BamHI sites, creating three restriction sites and encoding six His residues.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Chen BP, Hai T. Expression vectors for affinity purification and radiolabeling of proteins using Escherichia coli as host. Gene 139: 73-75, 1994. PubMed: 8112591

Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid