pJ3H (ATCC® 87187)

Applications: encodes an epitope tag for protein isolation or monitoringexpression vector  /  Depositors: J Chernoff

Permits and Restrictions

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Designations pJ3H
Depositors J Chernoff
Biosafety Level 1
Vector Information
Size (kb): 3.5000000000000000
Vector: pJ3H (plasmid)
Promoters: Promoter SV40 Early
Construction: pJ3omega
Marker(s):ampR
Construct size (kb): 3.5
Features: marker(s): ampR
other: SV40 t IVS
promoter: SV40 Early
replicon: SV40
replicon: pMB1
MCS: XbaI...BglII
terminator: SV40 T polyadenylation
epitope tag: hemagglutinin (HA)
Applications
encodes an epitope tag for protein isolation or monitoring
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.5; EcoRI--3.5; PvuI--1.95, 1.5.
Shuttle expression vector encoding an influenza hemagglutinin (HA) epitope tag, recognized by monoclonal antibody 12CA5, to facilitate protein isolation and monitoring.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sells MA, Chernoff J. Epitope-tag vectors for eukaryotic protein production. Gene 152: 187-189, 1995. PubMed: 7530674

Shipped freeze-dried
Product Sheet
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Product Sheet