p414 GAL1 (ATCC® 87328)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

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Designations p414 GAL1
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 5.4739999771118160
Vector: p414 GAL1 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pRS414, GAL1 promoter
Marker(s):TRP1,ampR
Construct size (kb): 5.473999977111816
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GAL1
MCS: XhoI...SpeI
terminator: CYC1
centromere: CEN6/ARSH4
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3, 1.8, 0.5; EcoRV--4.2, 1.5; HindIII--4.5, 1.3.
Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
Shipped freeze-dried
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Attachments Vector map for 87328