pREV-T3 (ATCC® 45011)

Organism: Avian reticuloendotheliosis virus (type C)  /  Clone Type: Clone  /  Depositors: HM Temin

Permits and Restrictions
Permits and Restrictions

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Designations pREV-T3
Species Avian reticuloendotheliosis virus (type C)
Depositors HM Temin
Vector
Construct size (kb): 9.359999656677246
Insert
DNA: genomic
Insert lengths(kb): 5.0
Tissue: DNA from chicken embryo fibroblasts
Gene product: [v-rel]
Insert Size (kb) 5.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid

Comments
MARKERS: Ap(r); Tc(r).
Unintegrated circular REV-T DNA. Constructed by inserting permuted REV-T SalI fragment (one LTR) into SalI site of pBR322
MARKERS: Ap(r); Tc(r).
A full-length clone that is infectious after SalI digestion and ligation.
The insert is missing one LTR.
The insert sequence starts at the SalI site preceding ATG for gag and continues beyond the LTR at the right.
References

Chen IS, Temin HM. Substitution of 5' helper virus sequences into non-rel portion of reticuloendotheliosis virus strain T suppresses transformation of chicken spleen cells. Cell 31: 111-120, 1982. PubMed: 6297744

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